Learning from intracellular metabolism: building molecular combinations

Metabolons, metabolic enzyme complexes in cells, promote and regulate metabolism efficiently by dynamically forming and dissociating enzyme complexes involved in metabolic pathways. Since the interactions of proteins and enzymes related to metabolism change in a temporo-spatial manner, the spatial organization of these enzyme complexes and their reaction characteristics remain unclear. To tackle this, I am developing molecular combinations in which functional molecules are positioned at the single-molecule level on DNA nanostructures. By constructing enzyme complexes with specific spatial arrangements in vitro and analyzing metabolic reactions while dynamically changing the configuration of the combinations, I aim to reveal the relationship between complex formation and reaction efficiency, shedding light on the mechanism of intracellular enzyme complexes’ structures and functions.

URL

https://researchmap.jp/lin-kyoto-2021?lang=en